Random mutagenesis and screening of complex glycoproteins: expression of human gonadotropins in Dictyostelium discoideum
نویسندگان
چکیده
منابع مشابه
Identification of glycoproteins, using lectins as probes, in plasma membranes from Dictyostelium discoideum and human erythrocytes.
The glycoproteins of plasma membranes from axenically grown Dictyostelium discoideum and human red blood cells (O+) were characterized according to their apparent molecular weights in sodium dodecyl sulfate-polyacrylamide gels and their ability to bind lectins. This was achieved by diffusing each of several fluorescein-conjugated lectins into sodium dodecyl sulfate-polyacrylamide gels which con...
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Electrophoretic variants which arise from amino acid substitutions, leading to charge differences between proteins are ubiquitous and have been used extensively for genetic analysis. Less well documented are polymorphisms in the size of proteins. Here we report that a group of glycoproteins, which share a common carbohydrate epitope, vary in size in different isolates of the cellular slime moul...
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Growth factors and their binding proteins are important proteins regulating mammalian cell proliferation and differentiation so there is considerable interest in producing them as recombinant proteins, especially in hosts that do not already produce a complex mixture of growth factors. Many growth factors require post-translational modifications making them unsuitable for production in Escheric...
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The recent publication of the apo-, closed-state 3D crystal structure of zebrafish (zf) P2X4.1 has not only revolutionized the P2X research field, but also highlighted the need for further crystal structures, of receptors in different activation states, so that we can gain a complete molecular understanding of ion channel function. zfP2X4.1 was selected as a 3D-crystallization candidate because...
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The eukaryote Dictyostelium discoideum is an attractive model organism for the study of cytoskeletal proteins and cell motility. The appearance and behavior of this cell closely resembles that of mammalian cells, but unlike mammalian cells, Dictyostelium offers the opportunity specifically to alter the cell physiology by molecular genetic approaches.
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 1999
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.13.6.639